2D-LC–Q-TOF Mass Spectrometry System
Two-dimensional liquid chromatography coupled with high-resolution Q-TOF mass spectrometry for the separation, identification and characterisation of complex samples.
Funded by: Enterprise Ireland
Manufacturer: Agilent Technologies
Available at CABR for industry collaborations
What is the 2D LC/MS system?
The 2D LC/MS system combines two-dimensional liquid chromatography with high-resolution quadrupole time-of-flight mass spectrometry.
Two-dimensional chromatography uses two complementary separation steps, providing greater separation capacity and selectivity than a conventional one-dimensional method. This is particularly valuable when analysing complex samples or compounds that are difficult to separate.
The Q-TOF mass spectrometer provides accurate-mass and MS/MS data that can support the detection, identification and characterisation of sample components. ESI and APCI ionisation options extend the range of compounds that may be analysed, subject to the instrument configuration and analytical method used.
Key Capabilities
Enhanced separation of complex mixtures using two-dimensional liquid chromatography
Accurate-mass measurement and MS/MS characterisation
Analysis of a broad range of small molecules, peptides and proteins
Targeted screening and quantification using suitable reference standards and analytical methods
Investigation of compounds that are unresolved or co-elute in conventional LC methods
Applications Across Sectors
Cosmetics
- Characterisation of selected bioactive compounds in ingredients and formulations
- Targeted quantification and comparative formulation studies
- Quality-control method development
Biopharmaceutical and Pharmaceutical
- Characterisation of active ingredients, impurities and selected matrix components
- Accurate-mass analysis of small molecules, peptides and proteins
- Targeted screening for specified contaminants
Food and Bioresources
- Identification and targeted quantification of selected bioactive compounds
- Characterisation of complex natural extracts
- Screening for specified contaminants, including selected mycotoxins and pesticides, subject to method detection limits
Medical Devices
- Characterisation of drug-release and leachable profiles from coatings and device materials
- Identification of selected released, stabilising or degradation-related compounds
The exact analytical approach, detection limits and reporting capability depend on the sample, target compounds and method-development requirements.
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Contact Details
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